Hotspot

trnK-UUU-trnQ-UUG

LSC · 4317-5401 · 1085 bp

Back to Myosurus

Pi 0.00463
MarkerSeek score 61.80
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1085
variable_sites
5
pi
0.00463
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 AAAGCCGAGTACTCTACCGT TTCGAGTCTTTCCCTCCCAT 50.0 50.0 58.2 58.0 1216-1222 bp 1.0 79.0
trnK-UUU-trnQ-UUG_p2 AAGCCGAGTACTCTACCGTT TTCGAGTCTTTCCCTCCCAT 50.0 50.0 58.2 58.0 1215-1221 bp 1.0 79.0
trnK-UUU-trnQ-UUG_p3 AAAGCCGAGTACTCTACCGT AAGATAAATGGGACGTCGCC 50.0 50.0 58.2 58.1 1194-1200 bp 1.0 79.0
trnK-UUU-trnQ-UUG_p4 AAGCCGAGTACTCTACCGTT AAGATAAATGGGACGTCGCC 50.0 50.0 58.2 58.1 1193-1199 bp 1.0 79.0
trnK-UUU-trnQ-UUG_p5 AAGCCGAGTACTCTACCGTT TTGCAGGTTCGAGTCTTTCC 50.0 50.0 58.2 58.1 1222-1228 bp 1.0 78.4