Hotspot

ycf1

SSC · 105983-111364 · 5382 bp

Back to Myosotis

Pi 0.00242

MarkerSeek score 43.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5382
variable_sites: 51
pi: 0.00242
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AACCCACTCGTCTGTCTGTA	CGACTGCCGTTATTGGTATT	50.0	45.0	58.0	55.9	445 bp	1.0	64.9
ycf1_p2	ACCCACTCGTCTGTCTGTAA	CGACTGCCGTTATTGGTATT	50.0	45.0	58.0	55.9	444 bp	1.0	64.9
ycf1_p3	AGGCAATCTTGGATCCGTTC	CGACTGCCGTTATTGGTATT	50.0	45.0	58.0	55.9	792 bp	1.0	64.0
ycf1_p4	GGCAATCTTGGATCCGTTCT	CGACTGCCGTTATTGGTATT	50.0	45.0	58.0	55.9	791 bp	1.0	64.0
ycf1_p5	TATCGAACCCACTCGTCTGT	CGACTGCCGTTATTGGTATT	50.0	45.0	58.2	55.9	450 bp	1.0	63.6