Hotspot

rpl32-ndhF

SSC · 121503-122203 · 701 bp

Back to Myosotis

Pi 0.00478

MarkerSeek score 69.20

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 701
variable_sites: 14
pi: 0.00478
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	GCTGTCCAATATCCCCTCCT	TCGGAAAAAGAAGAAGCCCC	55.0	50.0	58.6	58.1	832-907 bp	1.0	80.0
rpl32-ndhF_p2	GCTGTCCAATATCCCCTCCT	CAAGGGTATGATCCACGCAT	55.0	50.0	58.6	57.7	780-855 bp	1.0	78.8
rpl32-ndhF_p3	GCTGTCCAATATCCCCTCCT	AAAAGAAGAAGCCCCACTCC	55.0	50.0	58.6	57.7	827-902 bp	1.0	78.4
rpl32-ndhF_p4	AACGCTGTCCAATATCCCCT	TCGGAAAAAGAAGAAGCCCC	50.0	50.0	58.8	58.1	835-910 bp	1.0	78.2
rpl32-ndhF_p5	GCTGTCCAATATCCCCTCCT	AAGCAAGGGTATGATCCACG	55.0	50.0	58.6	57.7	783-858 bp	1.0	78.2