Hotspot

ycf1-rps15

SSC · 111365-111690 · 326 bp

Back to Myosotis

Pi 0.00806

MarkerSeek score 55.40

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 326
variable_sites: 8
pi: 0.00806
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	AATACCAATAACGGCAGTCG	ACTTGAAATTCAACTGATCCTTTGT	45.0	32.0	55.9	57.6	461-478 bp	1.0	44.5
ycf1-rps15_p2	AATACCAATAACGGCAGTCG	TGAAATTCAACTGATCCTTTGT	45.0	31.8	55.9	54.2	458-475 bp	1.0	44.5
ycf1-rps15_p3	AATACCAATAACGGCAGTCG	ACTTGAAATTCAACTGATCCTTTG	45.0	33.3	55.9	56.1	461-478 bp	1.0	44.5
ycf1-rps15_p4	AATACCAATAACGGCAGTCG	CTTGAAATTCAACTGATCCTTTGT	45.0	33.3	55.9	56.1	460-477 bp	1.0	44.5
ycf1-rps15_p5	AATACCAATAACGGCAGTCG	ACTTGAAATTCAACTGATCCT	45.0	33.3	55.9	53.0	461-478 bp	1.0	44.5