Hotspot

tRNA-Pro-psaJ

LSC · 64662-65216 · 555 bp

Back to Myosotis

Pi 0.009

MarkerSeek score 72.50

Primer available yes

Label tRNA-Pro-psaJ

Feature Metadata

label_name: tRNA-Pro-psaJ
feature_type: igs
region: LSC
length_bp: 555
variable_sites: 21
pi: 0.009
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Pro-psaJ_p1	ACCCGTGACATTTTGTACCC	AAACCCGCTAAAGCTCCAAA	50.0	45.0	58.1	58.0	676-747 bp	1.0	85.5
tRNA-Pro-psaJ_p2	CCCGTGACATTTTGTACCCA	AAACCCGCTAAAGCTCCAAA	50.0	45.0	58.1	58.0	675-746 bp	1.0	85.5
tRNA-Pro-psaJ_p3	ACAGGATTTGAACCCGTGAC	AAACCCGCTAAAGCTCCAAA	50.0	45.0	58.1	58.0	687-758 bp	1.0	85.4
tRNA-Pro-psaJ_p4	CAGGATTTGAACCCGTGACA	AAACCCGCTAAAGCTCCAAA	50.0	45.0	58.1	58.0	686-757 bp	1.0	85.4
tRNA-Pro-psaJ_p5	CCTCGTTACTGCCCTTTTGT	AAACCCGCTAAAGCTCCAAA	50.0	45.0	58.1	58.0	734-805 bp	1.0	85.3