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psbE-petL

LSC · 62739-63899 · 1161 bp

Back to Myosotis

Pi 0.00407

MarkerSeek score 46.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1161
variable_sites: 18
pi: 0.00407
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TAAGCTAAACCGGTGCTGAC	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.9	58.0	1332-1352 bp	1.0	81.2
psbE-petL_p2	ATTTACCCCACTTCCCTCCA	GCCGCTAGTAGAAAACCGAA	50.0	50.0	57.9	58.0	1872-1892 bp	1.0	80.9
psbE-petL_p3	ATTTACCCCACTTCCCTCCA	AAGCCGCTAGTAGAAAACCG	50.0	50.0	57.9	58.0	1874-1894 bp	1.0	80.9
psbE-petL_p4	ATTTACCCCACTTCCCTCCA	AGGGATCCGTTGAGATCGAT	50.0	50.0	57.9	58.0	2484-2506 bp	1.0	80.7
psbE-petL_p5	ATTTACCCCACTTCCCTCCA	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.9	58.0	2421-2443 bp	1.0	80.6