Hotspot

petA-psbJ

LSC · 61108-61946 · 839 bp

Back to Myosotis

Pi 0.00128

MarkerSeek score 60.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 839
variable_sites: 5
pi: 0.00128
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTTCAAGGCCTTTTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.5	57.6	967-980 bp	1.0	75.3
petA-psbJ_p2	CGTGTTCAAGGCCTTTTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.4	57.6	966-979 bp	1.0	74.9
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	957-970 bp	0.909091	74.6
petA-psbJ_p4	CGTTACGTGTTCAAGGCCTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.8	57.6	971-984 bp	1.0	73.4
petA-psbJ_p5	GACCCGTTACGTGTTCAAGG	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.9	57.6	975-988 bp	1.0	72.7