Hotspot

atpB-rbcL

LSC · 53661-54383 · 723 bp

Back to Myosotis

Pi 0.00282

MarkerSeek score 56.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 723
variable_sites: 7
pi: 0.00282
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GAAACCCCAGAACCAGAAGG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	801-816 bp	1.0	72.8
atpB-rbcL_p2	GGAAACCCCAGAACCAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	802-817 bp	1.0	72.8
atpB-rbcL_p3	GAAACCCCAGAACCAGAAGG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	800-815 bp	1.0	72.6
atpB-rbcL_p4	GGAAACCCCAGAACCAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	801-816 bp	1.0	72.6
atpB-rbcL_p5	ACCCCAGAACCAGAAGGAAT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.2	58.0	798-813 bp	1.0	71.6