Hotspot

ndhC-tRNA-Val

LSC · 49873-50587 · 715 bp

Back to Myosotis

Pi 0.00295

MarkerSeek score 60.10

Primer available yes

Label ndhC-tRNA-Val

Feature Metadata

label_name: ndhC-tRNA-Val
feature_type: igs
region: LSC
length_bp: 715
variable_sites: 10
pi: 0.00295
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-tRNA-Val_p1	CGGGGCTAAAACTCCTGAAA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.8	58.1	843-850 bp	1.0	79.5
ndhC-tRNA-Val_p2	CCTTGCTAATCGGGGCTAAA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	853-860 bp	1.0	78.4
ndhC-tRNA-Val_p3	ATCGGGGCTAAAACTCCTGA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.4	58.1	845-852 bp	1.0	77.8
ndhC-tRNA-Val_p4	AATCGGGGCTAAAACTCCTG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.6	58.1	846-853 bp	1.0	77.8
ndhC-tRNA-Val_p5	CGGGGCTAAAACTCCTGAAA	AGTGCATGTTGGGTCTTTGA	50.0	45.0	57.8	57.6	907-914 bp	1.0	77.3