Hotspot

tRNA-Thr-psbD

LSC · 30616-31899 · 1284 bp

Back to Myosotis

Pi 0.00636

MarkerSeek score 66.60

Primer available yes

Label tRNA-Thr-psbD

Feature Metadata

label_name: tRNA-Thr-psbD
feature_type: igs
region: LSC
length_bp: 1284
variable_sites: 27
pi: 0.00636
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Thr-psbD_p1	GTGGTAGAGTAACGCCATGG	CCGCAACCAGTCATCCATAA	55.0	50.0	58.1	58.0	1386-1427 bp	1.0	82.4
tRNA-Thr-psbD_p2	TCAGTGGTAGAGTAACGCCA	CCGCAACCAGTCATCCATAA	50.0	50.0	58.1	58.0	1389-1430 bp	1.0	82.2
tRNA-Thr-psbD_p3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1406-1447 bp	1.0	80.7
tRNA-Thr-psbD_p4	GGCGTAAGTCATCGGTTCAA	CCGCAACCAGTCATCCATAA	50.0	50.0	58.3	58.0	1363-1404 bp	1.0	80.7
tRNA-Thr-psbD_p5	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	1420-1461 bp	1.0	80.5