Hotspot

tRNA-Glu-tRNA-Thr

LSC · 29800-30543 · 744 bp

Back to Myosotis

Pi 0.00318

MarkerSeek score 64.80

Primer available yes

Label tRNA-Glu-tRNA-Thr

Feature Metadata

label_name: tRNA-Glu-tRNA-Thr
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 12
pi: 0.00318
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Glu-tRNA-Thr_p1	TTCGTAGTACCACCCTACCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.9	58.0	873-907 bp	1.0	83.9
tRNA-Glu-tRNA-Thr_p2	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	817-851 bp	1.0	83.9
tRNA-Glu-tRNA-Thr_p3	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	815-849 bp	1.0	83.9
tRNA-Glu-tRNA-Thr_p4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	794-828 bp	1.0	83.5
tRNA-Glu-tRNA-Thr_p5	TTCGTAGTACCACCCTACCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.9	58.1	852-886 bp	1.0	83.4