Hotspot

atpA-atpF

LSC · 11439-11505 · 67 bp

Back to Myosotis

Pi 0.0457

MarkerSeek score 84.60

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 67
variable_sites: 8
pi: 0.0457
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCGCCTACTTGAAGTAC	TTGGGGGCGCTAAAAGAAAT	55.0	45.0	58.1	58.1	198-214 bp	1.0	86.2
atpA-atpF_p2	ACTAATTTCGTCGGCTCGAA	TTGGGGGCGCTAAAAGAAAT	45.0	45.0	57.4	58.1	115-131 bp	1.0	84.2
atpA-atpF_p3	CGTCGCCTACTTGAAGTACA	TTGGGGGCGCTAAAAGAAAT	50.0	45.0	57.4	58.1	197-213 bp	1.0	82.0
atpA-atpF_p4	CCGTCGCCTACTTGAAGTAC	TGGGGGCGCTAAAAGAAATA	55.0	45.0	58.1	57.2	197-213 bp	1.0	80.3
atpA-atpF_p5	CCGTCGCCTACTTGAAGTAC	AATATTGGCATGTTGGGGGC	55.0	50.0	58.1	58.9	210-226 bp	1.0	79.6