Hotspot

rrn16

IRb · 101685-103175 · 1491 bp

Back to Mussaenda

Pi 0

MarkerSeek score 29.00

Primer available yes

Label rrn16

Feature Metadata

label_name: rrn16
feature_type: rRNA
region: IRb
length_bp: 1491
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16_p1	CGAATCCGCTTTGTCTACGA	GTGTGAAGCGGTGTCAAAC	50.0	52.6	58.1	57.5	1593 bp	1.0	67.0
rrn16_p2	CGAATCCGCTTTGTCTACGA	GGTGTGAAGCGGTGTCAAA	50.0	52.6	58.1	58.6	1594 bp	1.0	66.4
rrn16_p3	CGAATCCGCTTTGTCTACGA	TGTGAAGCGGTGTCAAACC	50.0	52.6	58.1	58.6	1592 bp	1.0	66.4
rrn16_p4	GAACTCCGGGCGAATATGAA	GTGTGAAGCGGTGTCAAAC	50.0	52.6	57.8	57.5	1654 bp	1.0	65.9
rrn16_p5	AACTCCGGGCGAATATGAAG	GTGTGAAGCGGTGTCAAAC	50.0	52.6	57.8	57.5	1653 bp	1.0	65.9