Hotspot

rrn16-trnI-GAU

IRb · 103176-103477 · 302 bp

Back to Mussaenda

Pi 0.0373
MarkerSeek score 76.10
Primer available yes
Label rrn16-trnI-GAU

Feature Metadata

label_name
rrn16-trnI-GAU
feature_type
igs
region
IRb
length_bp
302
variable_sites
11
pi
0.0373
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-trnI-GAU_p1 CAAGGTAGCCGTACTGGAAG GGCACAACGACGCAATTATC 55.0 50.0 57.7 58.2 389-396 bp 1.0 79.8
rrn16-trnI-GAU_p2 CAAGGTAGCCGTACTGGAAG CTATCCATGTGGCTGAGAGC 55.0 55.0 57.7 57.8 421-428 bp 1.0 79.7
rrn16-trnI-GAU_p3 AGTGACTGGAGTGAAGTCGT GGCACAACGACGCAATTATC 50.0 50.0 58.3 58.2 411-418 bp 1.0 79.5
rrn16-trnI-GAU_p4 AGTGACTGGAGTGAAGTCGT CTATCCATGTGGCTGAGAGC 50.0 55.0 58.3 57.8 443-450 bp 1.0 79.4
rrn16-trnI-GAU_p5 AAGTCGTAACAAGGTAGCCG CTATCCATGTGGCTGAGAGC 50.0 55.0 57.6 57.8 430-437 bp 1.0 79.1