Hotspot

rps4-trnT-UGU

LSC · 46707-47099 · 393 bp

Back to Mussaenda

Pi 0.0027

MarkerSeek score 60.60

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 393
variable_sites: 1
pi: 0.0027
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CTCCTAGGCGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	483-505 bp	1.0	82.3
rps4-trnT-UGU_p2	CTCCTAGGCGGCGTATTTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	451-473 bp	1.0	81.7
rps4-trnT-UGU_p3	CTCCTAGGCGGCGTATTTTT	TGGATGTTATATGAGCCCGC	50.0	50.0	58.1	57.5	507-529 bp	1.0	79.4
rps4-trnT-UGU_p4	CTCCTAGGCGGCGTATTTTT	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.1	57.5	491-513 bp	1.0	79.3
rps4-trnT-UGU_p5	CTCCTAGGCGGCGTATTTTT	CAGAGGTTAGAGCATCGCAT	50.0	50.0	58.1	57.5	481-503 bp	1.0	79.2