Hotspot

rps16-trnQ-UUG

LSC · 6212-7655 · 1444 bp

Back to Mussaenda

Pi 0.00346

MarkerSeek score 60.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1444
variable_sites: 5
pi: 0.00346
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1517 bp	1.0	78.5
rps16-trnQ-UUG_p2	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1531 bp	1.0	76.7
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	AAAAATCGAAATGGGGCGTG	45.0	45.0	58.1	57.6	1576 bp	1.0	76.2
rps16-trnQ-UUG_p4	GGATCGTGTCCTTCAAGTCG	AAAAATCGAAATGGGGCGTG	55.0	45.0	58.4	57.6	1590 bp	1.0	74.4
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1521 bp	1.0	73.8