Hotspot

trnK-UUU-rps16

LSC · 4277-5105 · 829 bp

Back to Mussaenda

Pi 0.00605

MarkerSeek score 54.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 829
variable_sites: 5
pi: 0.00605
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	964-966 bp	1.0	79.2
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.2	58.0	963-965 bp	1.0	79.2
trnK-UUU-rps16_p3	TCCTAGCCGCACTTAAAAGC	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.3	58.0	979-981 bp	1.0	78.4
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	TTTCCTTGAAAGGGGTGCTC	50.0	50.0	58.3	58.0	973-975 bp	1.0	78.3
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	950-952 bp	1.0	77.7