Hotspot

accD

LSC · 57101-58579 · 1479 bp

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Pi 0.0161

MarkerSeek score 70.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1479
variable_sites: 149
pi: 0.0161
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCAAATAGGGGGCAGGAAGA	ATGGGAGTTCTTGGTAGGCA	50.0	50.0	58.0	58.3	1537-1573 bp	0.897436	80.7
accD_p2	TCAAATAGGGGGCAGGAAGA	TGGGAGTTCTTGGTAGGCAT	50.0	50.0	58.0	58.3	1536-1572 bp	0.897436	80.7
accD_p3	TCATCAAATAGGGGGCAGGA	ATGGGAGTTCTTGGTAGGCA	50.0	50.0	58.1	58.3	1540-1576 bp	0.897436	80.1
accD_p4	TCATCAAATAGGGGGCAGGA	TGGGAGTTCTTGGTAGGCAT	50.0	50.0	58.1	58.3	1539-1575 bp	0.897436	80.1
accD_p5	TCAAATAGGGGGCAGGAAGA	AATGGGAGTTCTTGGTAGGC	50.0	50.0	58.0	57.2	1538-1574 bp	0.897436	76.9