Hotspot

ycf4-cemA

LSC · 59991-60582 · 592 bp

Back to Muscari

Pi 0.0142

MarkerSeek score 67.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 58
pi: 0.0142
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TATTTCTTGCGCGTACCGAT	CACCAGGGCAAAACCACTAT	45.0	50.0	57.8	58.1	515-965 bp	1.0	88.5
ycf4-cemA_p2	GAACAAAAAGCGGCCGAATT	CACCAGGGCAAAACCACTAT	45.0	50.0	58.2	58.1	539-989 bp	1.0	88.5
ycf4-cemA_p3	TATTTCTTGCGCGTACCGAT	ATGAGAGAGAGACCCACCAG	45.0	55.0	57.8	57.8	529-979 bp	1.0	87.9
ycf4-cemA_p4	GAACAAAAAGCGGCCGAATT	ATGAGAGAGAGACCCACCAG	45.0	55.0	58.2	57.8	553-1003 bp	1.0	87.8
ycf4-cemA_p5	GGAACCATTCCCTTGACTCG	CACCAGGGCAAAACCACTAT	55.0	50.0	58.3	58.1	590-1040 bp	0.923077	84.7