Hotspot

ndhC-trnV-UAC

LSC · 50319-51206 · 888 bp

Back to Muscari

Pi 0.0137
MarkerSeek score 69.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
888
variable_sites
98
pi
0.0137
unique_haplotype_count
36
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGCCAAAATAGGAATAGCACCT AGAAGGTCTACGGTTCGAGT 40.9 50.0 57.8 57.8 690-1099 bp 1.0 72.4
ndhC-trnV-UAC_p2 TGCCAAAATAGGAATAGCACCT AGCCCAATGCAACAAAATGC 40.9 45.0 57.8 58.5 772-1181 bp 1.0 69.9
ndhC-trnV-UAC_p3 GCCAAAATAGGAATAGCACCTG AGAAGGTCTACGGTTCGAGT 45.5 50.0 57.4 57.8 689-1098 bp 1.0 69.8
ndhC-trnV-UAC_p4 TGCCAAAATAGGAATAGCACCT GAAGGTCTACGGTTCGAGTC 40.9 55.0 57.8 57.5 689-1098 bp 1.0 69.7
ndhC-trnV-UAC_p5 TGCCAAAATAGGAATAGCACC AGAAGGTCTACGGTTCGAGT 42.9 50.0 56.3 57.8 690-1099 bp 0.974359 66.4