Hotspot

trnT-GGU-psbD

LSC · 32401-33487 · 1087 bp

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Pi 0.0104

MarkerSeek score 59.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1087
variable_sites: 95
pi: 0.0104
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCATCGACCCAGGAAGAATT	TCGCAGTTGAACAGATCGAG	50.0	50.0	58.0	58.0	1980-2225 bp	1.0	89.4
trnT-GGU-psbD_p2	GCATCGACCCAGGAAGAATT	CTGCTACACCAAAACTGGGT	50.0	50.0	58.0	58.0	2092-2337 bp	1.0	89.1
trnT-GGU-psbD_p3	GCATCGACCCAGGAAGAATT	CACCGTCACCATCTTCGAAT	50.0	50.0	58.0	58.0	2248-2493 bp	1.0	89.0
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	TCGCAGTTGAACAGATCGAG	55.0	50.0	58.1	58.0	1451-1580 bp	1.0	88.8
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CACCGTCACCATCTTCGAAT	55.0	50.0	58.1	58.0	1719-1848 bp	1.0	88.4