Hotspot

psbI-trnS-GCU

LSC · 8125-8251 · 127 bp

Back to Muscari

Pi 0.0414

MarkerSeek score 65.30

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 127
variable_sites: 43
pi: 0.0414
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GATCCGGGCCGTAATCCT	ATTGGGAGAGATGGCTGAGT	61.1	50.0	58.5	58.1	193-260 bp	0.974359	69.8
psbI-trnS-GCU_p2	GATCCGGGCCGTAATCCT	GGGTTCGAATCCCTCTCTCT	61.1	55.0	58.5	58.2	124-191 bp	0.974359	69.0
psbI-trnS-GCU_p3	GATCCGGGCCGTAATCCT	GTGGACTAAAGCGGCAGATT	61.1	50.0	58.5	58.3	175-242 bp	0.974359	68.6
psbI-trnS-GCU_p4	GATCCGGGCCGTAATCCT	TGGACTAAAGCGGCAGATTG	61.1	50.0	58.5	58.3	174-241 bp	0.974359	68.6
psbI-trnS-GCU_p5	GATCCGGGCCGTAATCCT	TTCGAATCCCTCTCTCTCCG	61.1	55.0	58.5	58.3	121-188 bp	0.974359	68.2