Hotspot

rps16-trnQ-UUG

LSC · 5795-7074 · 1280 bp

Back to Muscari

Pi 0.015

MarkerSeek score 71.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1280
variable_sites: 157
pi: 0.015
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	TATGTCCTTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	959-1555 bp	1.0	91.3
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	949-1545 bp	1.0	90.3
rps16-trnQ-UUG_p3	TATGTCCTTCAAGTCGCACG	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.0	57.8	963-1559 bp	1.0	90.2
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	953-1549 bp	1.0	89.2
rps16-trnQ-UUG_p5	TATGTCCTTCAAGTCGCACG	TACTCGGAGGTTCGAATCCT	50.0	50.0	58.0	57.6	965-1561 bp	1.0	88.5