Hotspot

matK-trnK-UUU

LSC · 3276-4030 · 755 bp

Back to Muscari

Pi 0.0076

MarkerSeek score 67.20

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 755
variable_sites: 52
pi: 0.0076
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TAGGAAGTGTTGTTGCCGAG	TGGGTTGCTAACTCAATGGT	50.0	45.0	57.8	57.0	757-855 bp	1.0	80.5
matK-trnK-UUU_p2	TAGGAAGTGTTGTTGCCGAG	TCTGGGTTGCTAACTCAATGG	50.0	47.6	57.8	57.9	759-857 bp	1.0	79.7
matK-trnK-UUU_p3	TAGGAAGTGTTGTTGCCGAG	CTGGGTTGCTAACTCAATGGT	50.0	47.6	57.8	58.2	758-856 bp	1.0	78.6
matK-trnK-UUU_p4	GGAAGTGTTGTTGCCGAGAT	TGGGTTGCTAACTCAATGGT	50.0	45.0	58.5	57.0	755-853 bp	1.0	78.0
matK-trnK-UUU_p5	AAGTGTTGTTGCCGAGATCT	TGGGTTGCTAACTCAATGGT	45.0	45.0	57.4	57.0	753-851 bp	0.948718	75.0