Hotspot

ycf1

IRb · 114671-120703 · 6033 bp

Back to Musa

Pi 0.0118
MarkerSeek score 53.20
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
IRb
length_bp
6033
variable_sites
264
pi
0.0118
unique_haplotype_count
46
primer_available
yes
alignment_reliability
0.97
markerseek_score
53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 ACACTGAAACCCGAATCGTT TCCAGATGCATCTCTCCTCT 45.0 50.0 57.8 57.2 796-1319 bp 0.9375 80.1
ycf1_p2 CGGAGCAAATGGTCTCGATT TCCAGATGCATCTCTCCTCT 50.0 50.0 58.3 57.2 1048-1571 bp 0.9375 78.7
ycf1_p3 CACACTGAAACCCGAATCGT TCCAGATGCATCTCTCCTCT 50.0 50.0 58.5 57.2 797-1320 bp 0.9375 78.1
ycf1_p4 TGAGGACTGGACCGATATGT TCCAGATGCATCTCTCCTCT 50.0 50.0 57.5 57.2 608-1018 bp 0.729167 70.3
ycf1_p5 TTCTCGGAGCAAATGGTCTC TCCAGATGCATCTCTCCTCT 50.0 50.0 57.6 57.2 1052-1480 bp 0.666667 65.2