Hotspot

rps19

IRa · 169390-169668 · 279 bp

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Pi 0
MarkerSeek score 19.30
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
IRa
length_bp
279
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
19.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 GGGTAGAGCCGGATCTAAGT AAACACTTGTTTTACTTTCAGT 55.0 27.3 58.0 52.7 879-882 bp 0.291667 12.3
rps19_p2 GGGTAGAGCCGGATCTAAGT AACACTTGTTTTACTTTCAGTT 55.0 27.3 58.0 52.7 878-881 bp 0.291667 12.3
rps19_p3 GGGTAGAGCCGGATCTAAGT ACACTTGTTTTACTTTCAGTTT 55.0 27.3 58.0 52.7 877-880 bp 0.291667 12.3
rps19_p4 TGGGTAGAGCCGGATCTAAG AACACTTGTTTTACTTTCAGTT 55.0 27.3 58.0 52.7 879-882 bp 0.291667 12.3
rps19_p5 TGGGTAGAGCCGGATCTAAG ACACTTGTTTTACTTTCAGTTT 55.0 27.3 58.0 52.7 878-881 bp 0.291667 12.3