Hotspot

ndhF

SSC · 123711-125921 · 2211 bp

Pi 0.0166

MarkerSeek score 52.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2211
variable_sites: 179
pi: 0.0166
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CAGTGGACGGTTAGGAACAC	GTACGCTTTTTGGGAACTGC	55.0	50.0	58.2	58.0	2722-2968 bp	0.416667	63.0
ndhF_p2	GGACGGTTAGGAACACCAAA	GTACGCTTTTTGGGAACTGC	50.0	50.0	57.7	58.0	2718-2964 bp	0.416667	62.6
ndhF_p3	TCAGTGGACGGTTAGGAACA	GTACGCTTTTTGGGAACTGC	50.0	50.0	58.3	58.0	2723-2969 bp	0.416667	62.4
ndhF_p4	CTTGGAGCGGGTGTAAAAGA	GTACGCTTTTTGGGAACTGC	50.0	50.0	57.8	58.0	2806-2907 bp	0.354167	58.7
ndhF_p5	TCTTGGAGCGGGTGTAAAAG	GTACGCTTTTTGGGAACTGC	50.0	50.0	57.8	58.0	2807-2908 bp	0.354167	58.7