Hotspot

ndhA

SSC · 133297-135449 · 2153 bp

Back to Musa

Pi 0.014
MarkerSeek score 57.20
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2153
variable_sites
142
pi
0.014
unique_haplotype_count
34
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 AGAATATCGTTATCGTATTCTGT TGGGAGAGGTTGATCGTTGA 30.4 50.0 53.0 58.4 2198-2869 bp 0.979167 47.7
ndhA_p2 AGAATATCGTTATCGTATTCTGT GTCCACCCGGCTTCATAAAT 30.4 50.0 53.0 57.9 2297-2968 bp 0.979167 47.3
ndhA_p3 AGAATATCGTTATCGTATTCTGTT GTCCACCCGGCTTCATAAAT 29.2 50.0 53.8 57.9 2297-2968 bp 0.979167 47.3
ndhA_p4 TGAATCTAGAATATCGTTATCGT GTCCACCCGGCTTCATAAAT 30.4 50.0 52.8 57.9 2304-2975 bp 0.979167 47.3
ndhA_p5 TCTAGAATATCGTTATCGTATTCTGT GTCCACCCGGCTTCATAAAT 30.8 50.0 55.5 57.9 2300-2971 bp 0.979167 47.3