Hotspot

ndhA-ndhF

IRb · 123193-123710 · 518 bp

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Pi 0.0386
MarkerSeek score 61.50
Primer available yes
Label ndhA-ndhF

Feature Metadata

label_name
ndhA-ndhF
feature_type
igs
region
IRb
length_bp
518
variable_sites
83
pi
0.0386
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.94
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA-ndhF_p1 CGCCTCATTTTAGCCGATCT GGGTTGGCATTGCAAGTTTT 50.0 45.0 58.1 58.0 565-1049 bp 0.666667 77.1
ndhA-ndhF_p2 TCGCCTCATTTTAGCCGATC GGGTTGGCATTGCAAGTTTT 50.0 45.0 58.1 58.0 566-1050 bp 0.666667 77.0
ndhA-ndhF_p3 TGCAGCGATACAACAACGTA GGGTTGGCATTGCAAGTTTT 45.0 45.0 57.9 58.0 761-1245 bp 0.666667 76.1
ndhA-ndhF_p4 GTCCACCCGGCTTCATAAAT GGGTTGGCATTGCAAGTTTT 50.0 45.0 57.9 58.0 1045-1529 bp 0.666667 74.9
ndhA-ndhF_p5 AACAACGTATTGGGCCTGAA GGGTTGGCATTGCAAGTTTT 45.0 45.0 57.7 58.0 750-1234 bp 0.666667 74.5