Hotspot

rpl22-rps19

IRb · 88276-88420 · 145 bp

Back to Musa

Pi 0.0856
MarkerSeek score 65.50
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
IRb
length_bp
145
variable_sites
51
pi
0.0856
unique_haplotype_count
27
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 CGCGCTTTGAAAACAGACAT GTTATCCTGCGCTTGGAAGA 45.0 50.0 58.0 58.0 632-1241 bp 1.0 91.5
rpl22-rps19_p2 CGCGCTTTGAAAACAGACAT TAGTAACTTGGTCTCGGGCA 45.0 50.0 58.0 58.1 400-780 bp 1.0 91.1
rpl22-rps19_p3 TGCTGCTGCAGAATAAACCA GTTATCCTGCGCTTGGAAGA 45.0 50.0 58.1 58.0 747-1356 bp 1.0 91.1
rpl22-rps19_p4 TTCGCGCTTTGAAAACAGAC GTTATCCTGCGCTTGGAAGA 45.0 50.0 57.9 58.0 634-1243 bp 1.0 91.0
rpl22-rps19_p5 TGCTGCTGCAGAATAAACCA TAGTAACTTGGTCTCGGGCA 45.0 50.0 58.1 58.1 515-895 bp 1.0 90.7