Hotspot

trnT-UGU-trnL-UAA

LSC · 49160-49940 · 781 bp

Back to Musa

Pi 0.0261
MarkerSeek score 63.00
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
781
variable_sites
76
pi
0.0261
unique_haplotype_count
31
primer_available
yes
alignment_reliability
0.93
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 AGAGAAAAAGCCGGCTATCG GCTTCCATTGAGTCTCTGCA 50.0 50.0 58.1 57.9 1019-1364 bp 0.666667 73.6
trnT-UGU-trnL-UAA_p2 CGATAGAGAAAAAGCCGGCT GCTTCCATTGAGTCTCTGCA 50.0 50.0 58.1 57.9 1023-1368 bp 0.666667 73.6
trnT-UGU-trnL-UAA_p3 AGAGAAAAAGCCGGCTATCG CGGATTCCTGCTACCAACAA 50.0 50.0 58.1 57.9 1067-1412 bp 0.666667 73.5
trnT-UGU-trnL-UAA_p4 CGATAGAGAAAAAGCCGGCT CGGATTCCTGCTACCAACAA 50.0 50.0 58.1 57.9 1071-1416 bp 0.666667 73.5
trnT-UGU-trnL-UAA_p5 TCGATAGAGAAAAAGCCGGC GCTTCCATTGAGTCTCTGCA 50.0 50.0 58.1 57.9 1024-1369 bp 0.666667 73.5