Hotspot

atpH-atpI

LSC · 14921-16055 · 1135 bp

Back to Musa

Pi 0.015
MarkerSeek score 72.50
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1135
variable_sites
98
pi
0.015
unique_haplotype_count
37
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT TTTTTGCAACGTTAGCTGCG 45.0 45.0 58.0 58.5 1026-1580 bp 1.0 85.6
atpH-atpI_p2 ATAACGGAAGCGGCAGAAAT TAGGTGAATCCATGGAGGGT 45.0 50.0 58.0 57.4 999-1553 bp 1.0 85.3
atpH-atpI_p3 TACCTTCTACAGCTTGGCCT TTTTTGCAACGTTAGCTGCG 50.0 45.0 58.0 58.5 1109-1663 bp 1.0 85.2
atpH-atpI_p4 TACCTTCTACAGCTTGGCCT TAGGTGAATCCATGGAGGGT 50.0 50.0 58.0 57.4 1082-1636 bp 1.0 84.9
atpH-atpI_p5 TACCTTGACCAACTCCAGGT TTTTTGCAACGTTAGCTGCG 50.0 45.0 57.9 58.5 1082-1636 bp 1.0 84.8