Hotspot

trnG-UCC-trnR-UCU

LSC · 10743-10933 · 191 bp

Back to Musa

Pi 0.0523
MarkerSeek score 66.80
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
191
variable_sites
39
pi
0.0523
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.95
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AAGTGATCAAGCAACGTCGA AGAAGACCTCTGTCCTATCCA 45.0 47.6 57.9 56.9 157-343 bp 1.0 74.4
trnG-UCC-trnR-UCU_p2 AAGTGATCAAGCAACGTCGA AGGTTTAGAAGACCTCTGTCCT 45.0 45.5 57.9 57.9 163-349 bp 1.0 73.7
trnG-UCC-trnR-UCU_p3 GCAACGTCGACTATAACCCC AGAAGACCTCTGTCCTATCCA 55.0 47.6 58.4 56.9 147-333 bp 1.0 72.1
trnG-UCC-trnR-UCU_p4 GCAACGTCGACTATAACCCC AGGTTTAGAAGACCTCTGTCCT 55.0 45.5 58.4 57.9 153-339 bp 1.0 71.4
trnG-UCC-trnR-UCU_p5 AGCAACGTCGACTATAACCC AGAAGACCTCTGTCCTATCCA 50.0 47.6 57.4 56.9 148-334 bp 1.0 71.0