Hotspot

ndhF

SSC · 114148-116376 · 2229 bp

Back to Murraya

Pi 0.0152

MarkerSeek score 53.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2229
variable_sites: 66
pi: 0.0152
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TATCCGCCGAAGAAGATCCT	AAGAACCGGCGAATCAGAAA	50.0	45.0	58.0	57.8	2516-2564 bp	1.0	83.0
ndhF_p2	CAGGAACAAGAGGTATCCGC	AAGAACCGGCGAATCAGAAA	55.0	45.0	58.1	57.8	2529-2577 bp	1.0	82.6
ndhF_p3	GGTATCCGCCGAAGAAGATC	AAGAACCGGCGAATCAGAAA	55.0	45.0	57.9	57.8	2518-2566 bp	1.0	82.5
ndhF_p4	GTATCCGCCGAAGAAGATCC	AAGAACCGGCGAATCAGAAA	55.0	45.0	57.9	57.8	2517-2565 bp	1.0	82.5
ndhF_p5	TATCCGCCGAAGAAGATCCT	GTGCCAAGATGAACGGGTTA	50.0	50.0	58.0	58.2	2990 bp	0.25	48.5