Hotspot

psaC-ndhE

SSC · 121447-121681 · 235 bp

Back to Murraya

Pi 0.0376

MarkerSeek score 70.50

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 235
variable_sites: 16
pi: 0.0376
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TGCTTTACATCCGTCCCAAG	CGTAACAGAAAATCGACGCG	50.0	50.0	57.9	58.0	390-396 bp	1.0	82.9
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	CGTAACAGAAAATCGACGCG	50.0	50.0	58.1	58.0	384-390 bp	1.0	82.8
psaC-ndhE_p3	TCTAATACATCCGTGGGGCA	CGTAACAGAAAATCGACGCG	50.0	50.0	58.2	58.0	362-368 bp	1.0	82.4
psaC-ndhE_p4	TGCTTTACATCCGTCCCAAG	CGACGCGTATCAATCAATCG	50.0	50.0	57.9	57.5	377-383 bp	1.0	79.7
psaC-ndhE_p5	ACATCCGTCCCAAGGTATCA	CGACGCGTATCAATCAATCG	50.0	50.0	58.1	57.5	371-377 bp	1.0	79.6