Hotspot

ccsA-ndhD

SSC · 119170-119539 · 370 bp

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Pi 0.0471
MarkerSeek score 75.50
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
370
variable_sites
29
pi
0.0471
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.83
markerseek_score
75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGATATGCTATTTCGGAGTCA CGTTCTCTCACTATCAGCGG 40.9 55.0 56.8 57.9 442-494 bp 1.0 62.4
ccsA-ndhD_p2 TGGATATGCTATTTCGGAGTCA CTCACTATCAGCGGACAAGG 40.9 55.0 56.8 57.8 436-488 bp 1.0 61.7
ccsA-ndhD_p3 TGGATATGCTATTTCGGAGTCA TCTCTCACTATCAGCGGACA 40.9 50.0 56.8 57.6 439-491 bp 1.0 60.0
ccsA-ndhD_p4 TGGATATGCTATTTCGGAGTCAA CGTTCTCTCACTATCAGCGG 39.1 55.0 57.5 57.9 442-494 bp 1.0 59.8
ccsA-ndhD_p5 TTGGATATGCTATTTCGGAGTCA CGTTCTCTCACTATCAGCGG 39.1 55.0 57.5 57.9 443-495 bp 1.0 59.8