Hotspot

rpl36-rps8

LSC · 83140-83542 · 403 bp

Back to Murraya

Pi 0.0282

MarkerSeek score 66.30

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 403
variable_sites: 22
pi: 0.0282
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TGCGGATCAGTCGACATTTT	GCGGCTCGACTAGAAAGAAT	45.0	50.0	57.9	57.8	509-524 bp	1.0	82.0
rpl36-rps8_p2	TGCGGATCAGTCGACATTTT	CGGGGTATAATGACAGACCG	45.0	55.0	57.9	57.6	530-545 bp	1.0	80.1
rpl36-rps8_p3	CTGCGGATCAGTCGACATTT	GCGGCTCGACTAGAAAGAAT	50.0	50.0	58.4	57.8	510-525 bp	1.0	79.9
rpl36-rps8_p4	TATAATCCGTCCCCTCCTGC	GCGGCTCGACTAGAAAGAAT	55.0	50.0	58.4	57.8	526-541 bp	1.0	79.8
rpl36-rps8_p5	CTGCGGATCAGTCGACATTT	CGGGGTATAATGACAGACCG	50.0	55.0	58.4	57.6	531-546 bp	1.0	78.0