Hotspot

petA-psbJ

LSC · 65987-67089 · 1103 bp

Back to Murraya

Pi 0.0237

MarkerSeek score 71.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1103
variable_sites: 50
pi: 0.0237
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCATCTGTTGTTTTGGCACA	CCGGATTAGGTTCGTCCTTG	45.0	55.0	57.8	58.1	1210-1251 bp	1.0	83.5
petA-psbJ_p2	TTGGCATCTGTTGTTTTGGC	CCGGATTAGGTTCGTCCTTG	45.0	55.0	57.8	58.1	1213-1254 bp	1.0	83.4
petA-psbJ_p3	GCATCTGTTGTTTTGGCACA	TCCGGATTAGGTTCGTCCTT	45.0	50.0	57.8	58.1	1211-1252 bp	1.0	82.9
petA-psbJ_p4	TTGGCATCTGTTGTTTTGGC	TCCGGATTAGGTTCGTCCTT	45.0	50.0	57.8	58.1	1214-1255 bp	1.0	82.7
petA-psbJ_p5	GGCATCTGTTGTTTTGGCAC	CCGGATTAGGTTCGTCCTTG	50.0	55.0	58.5	58.1	1211-1252 bp	1.0	81.3