Hotspot

ycf4-cemA

LSC · 63340-64083 · 744 bp

Back to Murraya

Pi 0.0214

MarkerSeek score 67.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 31
pi: 0.0214
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGACTCCGCGAGAAATTGAG	TAAGGGGAATGGATGCGTTC	50.0	50.0	58.0	57.7	849-865 bp	1.0	82.4
ycf4-cemA_p2	TGAGAATTTGACTCCGCGAG	TAAGGGGAATGGATGCGTTC	50.0	50.0	58.0	57.7	857-873 bp	1.0	82.3
ycf4-cemA_p3	TGACTCCGCGAGAAATTGAG	CTAAGGGGAATGGATGCGTT	50.0	50.0	58.0	57.7	850-866 bp	1.0	82.3
ycf4-cemA_p4	TGAGAATTTGACTCCGCGAG	CTAAGGGGAATGGATGCGTT	50.0	50.0	58.0	57.7	858-874 bp	1.0	82.3
ycf4-cemA_p5	TGACTCCGCGAGAAATTGAG	GGATCCACCAGGGCAAAAAT	50.0	50.0	58.0	58.4	897-913 bp	1.0	81.4