Hotspot

psbC-trnS-TGA

LSC · 38150-38403 · 254 bp

Back to Murraya

Pi 0.0296

MarkerSeek score 68.90

Primer available yes

Label psbC-trnS-TGA

Feature Metadata

label_name: psbC-trnS-TGA
feature_type: igs
region: LSC
length_bp: 254
variable_sites: 12
pi: 0.0296
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-TGA_p1	GCAGCAGGGTTTGAAAAAGG	GGTTGATAGCTCCGGTCTTG	50.0	55.0	58.1	58.1	393-400 bp	1.0	83.4
psbC-trnS-TGA_p2	GCAGCAGGGTTTGAAAAAGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.1	58.2	339-346 bp	1.0	82.9
psbC-trnS-TGA_p3	CGTAGGTCACTTATGGCACG	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.4	58.1	433-440 bp	1.0	80.4
psbC-trnS-TGA_p4	GCAGCAGGGTTTGAAAAAGG	TGGTTGATAGCTCCGGTCTT	50.0	50.0	58.1	58.4	394-401 bp	1.0	80.4
psbC-trnS-TGA_p5	GCAGCAGGGTTTGAAAAAGG	TAGCTCCGGTCTTGAAAACC	50.0	50.0	58.1	57.5	387-394 bp	1.0	80.2