Hotspot

trnD-GTC-trnY-GTA

LSC · 33077-33562 · 486 bp

Back to Murraya

Pi 0.0293

MarkerSeek score 74.00

Primer available yes

Label trnD-GTC-trnY-GTA

Feature Metadata

label_name: trnD-GTC-trnY-GTA
feature_type: igs
region: LSC
length_bp: 486
variable_sites: 26
pi: 0.0293
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GTC-trnY-GTA_p1	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	534-556 bp	1.0	82.1
trnD-GTC-trnY-GTA_p2	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	533-555 bp	1.0	82.1
trnD-GTC-trnY-GTA_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	555-577 bp	1.0	81.1
trnD-GTC-trnY-GTA_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	554-576 bp	1.0	80.9
trnD-GTC-trnY-GTA_p5	CGGTGCTCTGACCAATTGAA	CGGACTGTAAATTCGTTGGC	50.0	50.0	58.5	57.5	550-572 bp	1.0	79.7