Hotspot

petN-psbM

LSC · 30422-31707 · 1286 bp

Back to Murraya

Pi 0.0305

MarkerSeek score 71.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1286
variable_sites: 74
pi: 0.0305
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	AAAACGGTCAGTCAAAGTGA	50.0	40.0	57.6	55.4	1317-1354 bp	1.0	64.6
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	AAAAACGGTCAGTCAAAGTGA	50.0	38.1	57.6	56.2	1318-1355 bp	1.0	62.9
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	AAACGGTCAGTCAAAGTGAT	50.0	40.0	57.6	55.2	1316-1353 bp	1.0	62.7
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	AACGGTCAGTCAAAGTGATT	50.0	40.0	57.6	55.2	1315-1352 bp	1.0	62.7
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	AAACGGTCAGTCAAAGTGATT	50.0	38.1	57.6	56.0	1316-1353 bp	1.0	61.2