Hotspot

trnS-GCT-trnR-TCT

LSC · 8914-10739 · 1826 bp

Back to Murraya

Pi 0.0293

MarkerSeek score 72.80

Primer available yes

Label trnS-GCT-trnR-TCT

Feature Metadata

label_name: trnS-GCT-trnR-TCT
feature_type: igs
region: LSC
length_bp: 1826
variable_sites: 105
pi: 0.0293
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCT-trnR-TCT_p1	CCGACGCTTTAATCCACTCA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1893-1963 bp	1.0	68.5
trnS-GCT-trnR-TCT_p2	CCGACGCTTTAATCCACTCA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1899-1969 bp	1.0	67.8
trnS-GCT-trnR-TCT_p3	ACGGAAAGAGAGGGATTCGA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.2	56.9	1951-2021 bp	1.0	67.2
trnS-GCT-trnR-TCT_p4	ACGGAAAGAGAGGGATTCGA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.2	57.9	1957-2027 bp	1.0	66.5
trnS-GCT-trnR-TCT_p5	ACGCTTTAATCCACTCAGCC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.3	56.9	1890-1960 bp	1.0	66.5