Hotspot

trnN-AAC-trnR-CGU

IRa · 129667-130296 · 630 bp

Back to Murbeckiella

Pi 0.00476

MarkerSeek score 61.50

Primer available yes

Label trnN-AAC-trnR-CGU

Feature Metadata

label_name: trnN-AAC-trnR-CGU
feature_type: igs
region: IRa
length_bp: 630
variable_sites: 3
pi: 0.00476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-AAC-trnR-CGU_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	771 bp	1.0	78.6
trnN-AAC-trnR-CGU_p2	CCGTTGTTCCTCAGTAGCTC	GAACAGATCCAGTGGAGACG	55.0	55.0	58.0	57.7	808 bp	1.0	78.0
trnN-AAC-trnR-CGU_p3	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	812 bp	1.0	77.8
trnN-AAC-trnR-CGU_p4	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	722 bp	1.0	77.4
trnN-AAC-trnR-CGU_p5	TGGGTTGAGAACGGGAATTG	GAACAGATCCAGTGGAGACG	50.0	55.0	58.1	57.7	849 bp	1.0	77.2