Hotspot

rbcL-accD

LSC · 56112-56802 · 691 bp

Back to Murbeckiella

Pi 0.0145

MarkerSeek score 67.50

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 691
variable_sites: 10
pi: 0.0145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGTGAAGCTTGCAAATGGAG	AGTCTTTTCACCAGGAGCAA	50.0	45.0	58.0	57.0	896-898 bp	1.0	72.7
rbcL-accD_p2	CGTGAAGCTTGCAAATGGAG	CACCAGGAGCAAAACTATCCA	50.0	47.6	58.0	57.9	888-890 bp	1.0	72.4
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	AGTCTTTTCACCAGGAGCAA	55.0	45.0	57.7	57.0	879-881 bp	1.0	70.6
rbcL-accD_p4	CGTGAAGCTTGCAAATGGAG	AGTCTTTTCACCAGGAGCAAA	50.0	42.9	58.0	57.7	896-898 bp	1.0	70.6
rbcL-accD_p5	AAGCTTGCAAATGGAGTCCT	AGTCTTTTCACCAGGAGCAA	45.0	45.0	57.7	57.0	892-894 bp	1.0	70.5