Hotspot

trnM-AUG-atpE

LSC · 51846-51984 · 139 bp

Back to Murbeckiella

Pi 0.036

MarkerSeek score 73.20

Primer available yes

Label trnM-AUG-atpE

Feature Metadata

label_name: trnM-AUG-atpE
feature_type: igs
region: LSC
length_bp: 139
variable_sites: 5
pi: 0.036
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-AUG-atpE_p1	GGCAGGAGTCATTGGTTCAA	AGTCGAGGCTCTCAATACGA	50.0	50.0	58.1	58.0	199-202 bp	1.0	81.0
trnM-AUG-atpE_p2	GGCAGGAGTCATTGGTTCAA	ACGAGTCGAGGCTCTCAATA	50.0	50.0	58.1	58.0	202-205 bp	1.0	80.9
trnM-AUG-atpE_p3	TTGCTTTCATACGGCAGGAG	AGTCGAGGCTCTCAATACGA	50.0	50.0	58.3	58.0	211-214 bp	1.0	79.5
trnM-AUG-atpE_p4	TTGCTTTCATACGGCAGGAG	ACGAGTCGAGGCTCTCAATA	50.0	50.0	58.3	58.0	214-217 bp	1.0	79.4
trnM-AUG-atpE_p5	CATACGGCAGGAGTCATTGG	AGTCGAGGCTCTCAATACGA	55.0	50.0	58.4	58.0	204-207 bp	1.0	78.3