Hotspot

rps4-trnT-ACA

LSC · 45584-45990 · 407 bp

Back to Murbeckiella

Pi 0.00259

MarkerSeek score 61.00

Primer available yes

Label rps4-trnT-ACA

Feature Metadata

label_name: rps4-trnT-ACA
feature_type: igs
region: LSC
length_bp: 407
variable_sites: 1
pi: 0.00259
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-ACA_p1	CGGAGCGTGATTGGTTTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	564-585 bp	1.0	82.0
rps4-trnT-ACA_p2	CGGAGCGTGATTGGTTTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	532-553 bp	1.0	81.3
rps4-trnT-ACA_p3	TTACGGAGCGTGATTGGTTT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.8	57.9	567-588 bp	1.0	80.6
rps4-trnT-ACA_p4	TTTACGGAGCGTGATTGGTT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.8	57.9	568-589 bp	1.0	80.6
rps4-trnT-ACA_p5	TTTTACGGAGCGTGATTGGT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.8	57.9	569-590 bp	1.0	80.6