Hotspot

trnE-GAA-trnT-ACC

LSC · 30373-31192 · 820 bp

Back to Murbeckiella

Pi 0.00371

MarkerSeek score 62.20

Primer available yes

Label trnE-GAA-trnT-ACC

Feature Metadata

label_name: trnE-GAA-trnT-ACC
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 3
pi: 0.00371
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-GAA-trnT-ACC_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	950-961 bp	1.0	79.9
trnE-GAA-trnT-ACC_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	929-940 bp	1.0	79.4
trnE-GAA-trnT-ACC_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	926-937 bp	1.0	79.2
trnE-GAA-trnT-ACC_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	912-923 bp	1.0	79.1
trnE-GAA-trnT-ACC_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	891-902 bp	1.0	78.9