Hotspot

trnD-GAC-trnY-UAC

LSC · 29731-30156 · 426 bp

Back to Murbeckiella

Pi 0.00235

MarkerSeek score 53.50

Primer available yes

Label trnD-GAC-trnY-UAC

Feature Metadata

label_name: trnD-GAC-trnY-UAC
feature_type: igs
region: LSC
length_bp: 426
variable_sites: 1
pi: 0.00235
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GAC-trnY-UAC_p1	TGATGTATTGAATCCGCCGG	TGGCAATATGTCTACGCTGG	50.0	50.0	58.1	57.8	557 bp	1.0	77.3
trnD-GAC-trnY-UAC_p2	TGATGTATTGAATCCGCCGG	GGCAATATGTCTACGCTGGT	50.0	50.0	58.1	57.8	556 bp	1.0	77.2
trnD-GAC-trnY-UAC_p3	TGATGTATTGAATCCGCCGG	GGGGACGGACTGTAAATTCG	50.0	55.0	58.1	58.4	578 bp	1.0	76.3
trnD-GAC-trnY-UAC_p4	TGATGTATTGAATCCGCCGG	GGGACGGACTGTAAATTCGT	50.0	50.0	58.1	57.6	577 bp	1.0	76.2
trnD-GAC-trnY-UAC_p5	TGATGTATTGAATCCGCCGG	GATTCTTCCTGGGTCGATGC	50.0	55.0	58.1	58.4	611 bp	1.0	75.9