Hotspot

psbI-trnS-AGC

LSC · 7574-7664 · 91 bp

Back to Murbeckiella

Pi 0.022
MarkerSeek score 65.10
Primer available yes
Label psbI-trnS-AGC

Feature Metadata

label_name
psbI-trnS-AGC
feature_type
igs
region
LSC
length_bp
91
variable_sites
2
pi
0.022
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-AGC_p1 TCCTATCTAATGATCCAGGACGT TAGGAGAGATGGCTGAGTGG 43.5 55.0 58.0 57.9 225 bp 1.0 56.1
psbI-trnS-AGC_p2 TCCTATCTAATGATCCAGGACGT GGCTGAGTGGATTAAAGCGT 43.5 50.0 58.0 58.3 215 bp 1.0 54.7
psbI-trnS-AGC_p3 TCCTATCTAATGATCCAGGACGT TGGCTGAGTGGATTAAAGCG 43.5 50.0 58.0 58.3 216 bp 1.0 54.6
psbI-trnS-AGC_p4 TCCTATCTAATGATCCAGGACGT CGAGTTAATCGTACCGAGGG 43.5 55.0 58.0 57.6 175 bp 1.0 53.7
psbI-trnS-AGC_p5 CCTATCTAATGATCCAGGACGT TAGGAGAGATGGCTGAGTGG 45.5 55.0 56.6 57.9 224 bp 1.0 52.8